STUDIES IN STARCH AMYLASE VISCOSIMETRY

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Studies in Starch Amylase Viscosimetry

The viscosimetric method of estimating enzyme concentration was first developed by Northrop and Hussey (1). In studies of the effect of irradiation of trypsin and pepsin solutions by Hussey and Thompson (2) certain modifications of the original methods were employed in order to decrease the opportunity for personal influence in curve plotting, as in extrapolation for the initial viscosity of th...

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A Reproducible Standard Substrate in Starch-amylase Viscosimetry

A viscosimetric method of estimating enzyme concentration with special reference to amylase has been discussed in another communication 1 from this laboratory; and, in a later report, * the effects of variation in the concentration of starch from the standard lot and variation in lot of prepared soluble starch have been contrasted, indicating that only within a small range of variation of p (de...

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A Study of the Effects of Certain Variations in Preparation of a Starch Substrate in Amylase Viscosimetry

The results of employing different concentrations of starch and starch from different lots as substrates in the viscosimetric estimation of amylase concentration have been compared with the object of ultimate attainment of reproducible standards, independent of successive comparison.

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Amylase addition increases starch ruminal digestion in first-lactation cows fed high and low starch diets.

The objective of this study was to evaluate the effect of an exogenous amylase preparation on digestion of low- and high-starch diets in dairy cattle. Rumen and total-tract nutrient digestibility were measured in a 4×4 Latin square design with 28-d periods using 4 first-lactation cows cannulated at the rumen and duodenum. Corn silage-based diets had 20 or 30% starch, attained by changing the co...

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Thermostable, Raw-Starch-Digesting Amylase from Bacillus stearothermophilus.

An endospore-forming thermophilic bacterium, which produced amylase and was identified as Bacillus stearothermophilus, was isolated from soil. The amylase had an optimum temperature of 70 degrees C and strongly degraded wheat starch granules (93%) and potato starch granules (80%) at 60 degrees C.

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1935

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)75312-x